The human Elisa kit uses qualitative sandwich immunoassay technology and coats microplate strips with synthetic HEV peptide antigens. These peptides are peptides with strong antigenicity in the core amino acid sequence of Chinese HEV strains, which are derived from the virus The open reading frame 2 and open reading frame 3 of the strain. Add the sample or standard to the well and incubate. If HEVIgM antibodies are present, these antibodies will bind to the HEV polypeptide antigen and be fixed on it. Wash the plate to remove other non-specific antibodies and other components in the sample. Then add goat anti-human IgM-HRP (horseradish peroxidase) enzyme conjugate, after the second incubation, the enzyme conjugate will be combined with the HEVIgM antibody bound in the first incubation, wash the plate to remove unbound Enzyme conjugate, add TMB substrate solution, enzyme-substrate reaction will occur during the third incubation, only those containing the complex formed by HEVIgM antibody and enzyme conjugate will change color, add sulfuric acid solution Stop the reaction between the enzyme and the substrate, and measure the OD value at a wavelength of 450 nm. According to the test standard of the human Elisa kit, samples with an OD value greater than or equal to the Cut-Off value are considered positive for the initial test.
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