Outline of content: 1. Important considerations in the installation, commissioning and maintenance of liquid chromatography columns 1. Matching of column head types and stainless steel capillary fittings 2. Matching conversion of solvents 3. Equilibrium and aging before use of new columns 4. Use of pH Scope 5. Chromatographic column storage 2. Column pressure problems 1. After the packing is broken and used, there is packing powder generation 2. Particle pressure causes column pressure rise and countermeasures 1) Preventive measures 2) Troubleshooting 3. Column pressure caused by chemical pollutants Rise and countermeasures 1) Precautions 2) Trouble shooting 3. Peak shape problems 1. Trailing peaks 2. Peak forward delay 3. Other peak shape problems 4. Retention time problems 1. Reproducibility of retention time 2. Retention time drift 3. Reproducibility between columns 4. Reproducibility between batches 5. Life problems
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