Abstract: The Shanghai Institute of Microsystems and Information Technology of the Chinese Academy of Sciences has developed an ultra-sensitive explosive detector using the fluorescence multiplication effect of organic semiconductor polymers combined with integrated optical resonance fluorescence detection technology.
Explosives are usually analyzed by gas chromatography (GC) or liquid chromatography (LC) methods, but the analysis time of these methods is longer. Moreover, the GC method cannot be used for the analysis of thermally unstable compounds such as Tetral. Ultra High Performance Convergence Chromatography (UPC2TM) was successfully used to separate 14 explosives within 5 minutes.
Test Condition System: ACQUITY UPC2TM Detection: Photodiode Array (PDA)
PDA 3D channel: PDA, 205 to 400 nm, PDA 2D channel: 220 nm at 4.8 nm resolution (compensation 500 to 600 nm)
Column: ACQUITY UPC2HSS C18, 3.0 x 100 mm, 1.8 μm Mobile phase A: CO2 Mobile phase B: Acetonitrile washing solvent: 70:30 methanol / isopropanol Separation method: Start with 1% B, elute for 0.5 minutes, then The gradient was increased to 15% B within 2.5 minutes, eluted for 0.5 minutes, and then returned to the 1% B point within 0.1 minutes.
Flow rate: 2.0 mL / min UPC2 manager: 1500 psi Column temperature: 65 ° C Sample temperature: 10 ° C Injection volume: 1.0 μL Run time: 5.0 min Software: EmpowerTM3 Results and discussion Not only environmental labs need to conduct explosives Analysis, and the reference standard manufacturer must also analyze for the standard EPA method. The analysis time of the current method is relatively long, and it must be tested on an orthogonal chromatography column (ie, C18 and cyano or phenyl column). Achieve the required chromatographic resolution. Using UPC2 device can achieve the baseline resolution of 14 common explosives within 5 minutes.
Conclusion 14 kinds of explosives can be quickly analyzed. UPC2 is suitable for thermally unstable compounds such as Tetral, just as it separates different nitrotoluene isomers, it can also be used to separate nitroaromatic compounds.
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