FlexiTube siRNA Premix is ​​a typical time-saving and labor-saving reagent. It provides the siRNA of your gene of interest and efficient transfection reagents in a single tube format, which can be directly used for cell transfection without mixing. In traditional transfection experiments, the siRNA needs to be diluted first, and then mixed with the transfection reagent and incubated. In contrast, this premix can save a lot of time and labor. At the same time, the use of FlexiTube siRNA Premix also reduces the differences between experiments and between experiments, ensuring a high degree of consistency between different experiments.
In addition, FlexiTube siRNA Premix is ​​not just a simple mix, where the ratio of reagents to siRNA has been pre-optimized to ensure the highest transfection efficiency and gene silencing. You do not need to optimize the ratio of siRNA to reagents, you can quickly start the RNAi experiment, avoiding the lengthy experiment optimization process. QIAGEN currently provides FlexiTube siRNA Premix for human and mouse genes.
FlexiTube siRNA included in FlexiTube siRNA Premix is ​​designed using HP OnGuard siRNA Design to ensure its high specificity. The design of siRNA is based on artificial intelligence design procedures, combined with a large amount of RNAi experimental data. At the same time, the latest sequence database and patented homology analysis software were used to test the design of siRNA.
Everyone must be concerned about the efficiency of transfection, which of course also varies from cell to cell. As for the difficult primary cells and suspension cells, you can try to increase the dosage. Conversely, if you still want to reduce the final concentration of siRNA, of course, you only need to use a smaller amount of FlexiTube siRNA Premix. For example, use half of the recommended amount so that the final concentration of siRNA is 12.5 nM. Depending on the cell type, sometimes a final concentration of siRNA as low as 1 nM can produce efficient gene silencing.
Everyone knows that things that save time and effort generally do n’t save money, and of course FlexiTube siRNA Premix is ​​no exception. For FlexiTube siRNA alone, 1 nmol is about 930 yuan, while Premix's catalog price is 1770 yuan, which contains 0.75 nmol siRNA. Of course, you can't compare it directly, after all, you can save money on transfection reagents. If the laboratory can afford it, this is indeed a good option for saving trouble.
In addition, QIAGEN also brought a high-throughput version of HiPerFect transfection reagent – ​​HiPerFect HTS Reagent, which can transfect siRNA / miRNA with high throughput, which is suitable for pathway analysis, drug development and miRNA function research. For high-throughput experiments, easy operation, reliable results, and economical efficiency are all critical. HiPerFect HTS reagent can meet these points at the same time.
HiPerFect HTS reagent provides stable and reliable transfection, ensuring the consistency and reproducibility of high-throughput experiments. Its operation steps are also different, the purpose is to minimize the use of consumables, reduce the pipetting step, while saving resources and time, but also conducive to standardization.
The instructions provide transfection procedures for transfecting adherent cells in 96-well and 384-well plates. During operation, cells can be seeded and transfected on the same day. The specific process is that siRNA is first added to the well, then HiPerFect HTS reagent is added, and after the complex is formed, cells are added to the well.
In addition to the savings of plastic consumables, only a small amount of transfection reagents are used for each transfection, ensuring maximum economic benefits without compromising the reliability of the results. HiPerFect HTS reagent itself also has some advantages, such as high consistency between batches, and high stability after dilution. These properties are also very important for practical operation. For example, you can dilute it in the morning and use it for a whole day, and the transfection effect will not be discounted.
Currently, HiPerFect HTS reagent has been successfully transfected into some cell lines. In addition to the common 293, HeLa and other cell lines, primary cells such as HUVEC and NHDF can also be successfully transfected.
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