A research team at Kagoshima University in Japan used the mechanism of H1N1 influenza virus infection in humans as an entry point to develop a new method for rapid detection of influenza A virus.
The research team recently published a communique that says that the H1N1 influenza virus will bind to sugar chains on the surface of human cells and infect cells. Kagoshima University professor Sumida Taisheng and others used this as a breakthrough to study, artificially synthesizing this sugar chain on the surface of human cells, making it adhere to the surface of tiny gold particles.
In the experiment, the researchers mixed the saliva of patients with influenza A H1N1 virus with the gold particles and placed them in a centrifuge. The gold particles attached to the virus can be separated due to their larger mass, and the resulting virus concentration is higher.
At present, to determine whether someone is infected with the H1N1 influenza virus, we must first use a simple inspection tool to detect it, and then let the virus's genes multiply for polymerase chain reaction. However, because the influenza A virus has not proliferated to a sufficient amount in the infected person in the early stage of infection, the result of the current simple test may be negative. The new method will not cause such errors due to the high concentration of virus isolated.
The report said that the new method only takes about 30 minutes to determine whether the person is infected with the H1N1 influenza virus, and the current detection method, including polymerase chain reaction, takes longer. In addition, compared with taking samples from the depth of the subject's nasal cavity, the new method uses saliva and the subject has no discomfort.
Currently, the research team is cooperating with an enterprise in Hyogo Prefecture to develop a detection instrument using this new method.
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