Instructions:
1. Sample dilution is the same as national standard method 2. Inoculation
Beverage and drinking water use three dilutions of raw liquid, 1:10 and 1: 100? Solid food uses three dilutions of 1: 1, 1:10 and 1: 100.
Using a 1ml sterilized pipette, draw 1ml of the same dilution of the diluted solution onto the paper sheet in the bag and repeat three times. 3. Cultivation
Gently flatten the inoculated paper pieces (can be stacked) and incubate at 36 ° C for 15-24h to observe the results. 4. Result judgment
A purple spot appears on the paper, and a yellow circle around it is positive.? The paper is a kind of coloring, and it is negative if it grows aseptically.
The piece of paper is purple, with fuchsia spots, and the yellow circles around it are negative.? After acidic food inoculation, the paper sheet turns yellow, and no fuchsia spots are negative after cultivation
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