Human deoxypyridinoline ELISA detection kit scientific research detection steps

Test principle: The DPD kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards with known DPD concentrations and samples with unknown concentrations are added to microwell enzyme plates for detection. First, the DPD and biotin-labeled antibodies were incubated simultaneously. After washing, HRP labeled with avidin was added. After incubation and washing, the unbound enzyme conjugate is removed, and then substrates A and B are added to act simultaneously with the enzyme conjugate. Produce colors. The color depth is proportional to the DPD concentration in the sample.

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