Plant Efficiency Analyzer Principles and Application Examples
principle:
When dark-adapted plant leaves are excited by the excitation light provided by the plant efficiency analyzer Handy PEA, the leaf chlorophyll fluoresces, and the fluorescence is recorded by a monitor with high resolution. The monitor can record 100,000 fluorescence traces per second. Data, the chlorophyll fluorescence fast change curve (OJIP fluorescence induction curve) is accurately recorded, the instrument by analyzing the chlorophyll fluorescence-induced kinetics curve, get Fo, Fm, Fv, Fv/FmPIABS, φEo, ψO, RC/CSo, More than 50 fluorescence parameters such as ABS/CS, TR/CS, ET/CS, and DI/CS. These parameters reflect the photochemical activity of the photosynthetic apparatus PSII.
Figure 1 Plant Efficiency Analyzer and Dark Adaptation Clips (White)
Figure 2 Chlorophyll fluorescence induction kinetic curve (OJIP curve) measured with a plant efficiency analyzer
Application of Plant Efficiency Instrument in Physiological and Ecological Research
1 Fast determination of commonly used fluorescence parameters Fo, Fm, Fv/Fm
2 Study on photo-inhibition light destruction of plant PSII with Fv/Fm, and the daily change of Fv/Fm
As shown in the figure, the decrease in Fv/Fm indicates that the leaves are inhibited by light. From the above figure, we can see that, at 12:00, the Fv/Fm is significantly reduced, indicating that strong light at noon causes photoinhibition to the leaves, and 18:00 At the time, the Fv/Fm value returned to the level of 6:00 am, indicating that the photoinhibition was released. If at 18:00, the Fv/Fm value does not return to the 6:00 am level, indicating that light damage has occurred.
3 Application of fluorescence parameter PI in breeding superior varieties
Handy PEA plant efficiency meter has many advantages such as easy and quick measurement, easy to repeat, easy to carry and storage, so we can use Handy PEA to quickly select good breeds from offsprings, and can screen a large number of offspring groups. , and then further regular screening. The Performance Index (PI) of the photosynthetic index reflects the overall function of the PSII, and the PSII is the most vulnerable in adverse situations. Therefore, we can use the PI for rapid screening. PI is more sensitive than parameters such as Fv/Fm, which reflect PSII activity. Because Fv/Fm is the maximum photochemical efficiency, it is a potential function of PSII and does not change significantly under many stresses, while parameter PI reflects the overall function of PSII. As long as part of the PSII is hurt, the PI can react.
4 Study the status of PSII receptor side, donor side and reaction center
From the figure we can see that as the temperature rises, the fluorescence parameter Wk gradually increases, indicating that the high temperature damages the activity of the electron donor side of the PSII, that is, the oxygen releasing complex. The activity of the oxygen releasing complex was not harmed at a temperature below 35°C. As the temperature increased above 35°C, the activity of the oxygen releasing complex decreased. As the temperature increased, the fluorescence parameters RC/CS and ΨEo gradually decreased. It shows that high temperature damages the reaction center of PSII and the receptor side of PSII. Wk, RC/CS,
The ΨEo began to change at 37.5°C, 35°C, and 40°C, respectively, indicating that the high temperature first harmed the reaction center of PSII, followed by damaging the donor side (oxygen releasing complex), and finally damaging the receptor side of PSII. (Note: Not all plants are subjected to this rule under high temperature stress. Different plants have different sensitivity to temperature, and different parts of PSII have different levels of damage.)
5 Provide a deep theoretical foundation for research on photosynthesis
In addition, the plant efficiency analyzer has received more and more attention in adverse physiology. The plant efficiency analyzer also has many advantages such as large amount of information, simple and quick operation, easy and repeatable measurement, easy portability, large storage capacity, and low price. Therefore, we have reason to believe that the plant efficiency analyzer is bound to be used in scientific research. Widely used.
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